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A moderate threonine deficiency affects gene expression profile, paracellular permeability and glucose absorption capacity in the ileum of piglets ArchiMer
Hamard, Alice; Mazurais, David; Boudry, Gaelle; Le Huerou-luron, Isabelle; Seve, Bernard; Le Floc'H, Nathalie.
High dietary threonine extraction by the digestive tract suggests that threonine contributes to maintain gut physiology. In the present study, we evaluated the impact of a low (6.5 g of threonine/kg diet; LT group) or a control well-balanced threonine diet (9.3 g of threonine/kg diet; C group) given to piglets for 2 weeks on ileal permeability and Na+-dependant glucose absorption capacity in Ussing chambers. The paracellular permeability was significantly increased in the ileum of LT compared to C piglets (P=.017). The Na+-dependent glucose absorption capacity showed a nonsignificant increase in the LT piglets. In addition, we analysed ileal gene expression profiles in the LT and C groups using porcine multitissue cDNA microarrays. Compared to the C...
Tipo: Text Palavras-chave: Threonine; Small intestine; Paracellular permeability; Gene expression.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12531/9425.pdf
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Atrofia de neurônios do plexo mientérico do íleo de ratos submetidos à intensa carência de proteínas - doi: 10.4025/actascibiolsci.v34i2.6793 Biological Sciences
Moreira, Neide Martins; Universidade Estadual de Maringá; Muniz, Eliane; Universidade Paranaense; Hermes-Uliana, Catchia; Universidade Estadual de Maringá; Araújo, Eduardo José de Almeida; Universidade Estadual de Londrina; Sant'Ana, Debora de Mello Goncales; Universidade Estadual de Maringá.
Objetivou-se avaliar os efeitos da oferta de uma dieta contendo 4% de proteínas para ratos adultos, quanto aos aspectos morfométricos do plexo mientérico do íleo. Vinte animais foram distribuídos aleatoriamente em dois grupos: Controle (n = 10) que receberam ração comercial com 26% de proteína e Experimental (n = 10) alimentados com ração com teor proteico reduzido para 4%, durante 90 dias. Neurônios do plexo mientérico do íleo presentes em preparados totais foram evidenciados por intermédio da técnica de Giemsa e da NADH-diaforase. Tanto a população neuronal total, assim como a subpopulação NADH-diaforase positiva sofreram atrofia com redução da área do pericário, do núcleo e do citoplasma. 
Tipo: Estudos morfológicos Palavras-chave: Ciências Biológicas; Morfologia desnutrição proteica; Sistema nervoso entérico; Intestino delgado Morfologia protein malnutrition; Enteric nervous system; Small intestine.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6793
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Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats Anais da ABC (AABC)
PEREIRA,JOICE N.B.; MARI,RENATA B.; STABILLE,SANDRA R.; FARIA,HAROLDO G. DE; MOTA,THAIS F.M.; FERREIRA,WALTER M..
Aging is a biologic process characterized by progressive damage of structures and functions of organic systems. In gastrointestinal tract, it can involve enteric nervous system, which plays an important role in digestion and absorption of nutrients, causing hastening of intestinal transit thus reducing its absorptive function. Caloric restriction has been used in several studies with the intention of delaying deleterious effects of aging. This study aimed to evaluate the effects of caloric restriction on myenteric neurons of ileum by aging in rats. 30 Wistar rats were grouped as follows: GI (animals aged 6 months fed with normal diet), GII (animals aged 18 months fed with normal diet) and GIII (animals aged 18 months subject to 31% of caloric restriction)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; Caloric restriction; Myenteric plexus; Small intestine.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301471
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Distribution of mesenteric cranial artery in the small intestine of Procyon cancrivorus (Cuvier, 1798) (Mammalia, Procyonidae) - doi: 10.4025/actascibiolsci.v32i2.5839 Biological Sciences
Lima, Vanessa Morais; Universidade Federal de Goiás; Rezende, Ana Lúcia Souza; Universidade Federal de Goiás; Ferreira, Jussara Rocha; Universidade de Brasilía; Pereira, Kleber Fernando; Universidade Federal de Goiás.
The mesenteric artery is a high caliber vessel dependent on the abdominal artery, right below the emergence of the vessels destined for the liver and stomach. The present study aimed to describe the behavior of the arterial vessel in the small intestine of the crab-eating raccoon (Procyon cancrivorus). The animals were collected on highways (roadkill). Cannulation of the abdominal aorta, perfusion with warmed water (40°C) and stained latex injection (Neoprene 450, Dupont do Brasil and Sulvinil stain, Glassuret S.A) were performed, followed by formaldehyde fixation (10%). The dissection and photographic documents (Sony Cyber-shot, 8.1 megapixels) made it possible to systemize the arteries and define the vascular patterns of the viscera. It was...
Palavras-chave: 2.06.04.02-5 Anatomia Animal Procyon cancrivorus; Small intestine; Cranial mesenteric artery Anatomia Animal Macroscópica Procyon cancrivorus; Small intestine; Cranial mesenteric artery.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5839
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Effect of Broiler Breeder Age on the Intestinal Mucosa Development of the Embryos at 20 Days of Incubation Rev. Bras. Ciênc. Avic.
Maiorka,A; Silva,AVF; Santin,E; Bruno,LDG; Boleli,IC; Macari,M.
ABSTRACT This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeder age; Embryo; Microvillus; Small intestine; Villus.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600079
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Effect of Feeding Transcarpathian Zeolite on Gastrointestinal Morphology and Function in Broiler Chickens Rev. Bras. Ciênc. Avic.
Wawrzyniak,A; Kapica,M; Stępień-Pyśniak,D; Szewerniak,R; Olejarska,A; Jarosz,Ł.
ABSTRACT Many kinds of feed additives can be used in chicken diets to stimulate intestinal morphological maturation and, consequently, intestinal function. The purpose of this study was to evaluate the effect of zeolite on broiler performance, gut morphology and gut digestive enzyme activity. The one-day old broiler chickens Ross 308 were assigned randomly into 3 groups with 30 birds per treatment. The 3 dietary treatments were: basal diet only (control group), basal diet + 2% zeolite, basal diet + 3% zeolite. Morphological and morphometric intestinal assessments were conducted using a light microscope. There was observed increase in villus height, villus perimeter, villus section area and crypt depth throughout duodenum and mid jejunum of the broilers fed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestive enzyme; Histology; Small intestine; Zeolite.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400737
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Effects of Dietary Hot Pepper Waste Powder on Gut Health and Small Intestine Properties in Japanese Quails Rev. Bras. Ciênc. Avic.
Filik,G; Coşkun,I; Tekin,OK; Filik,AG.
ABSTRACT The present study was conducted to evaluate whether dietary hot pepper waste powder (HPWP) would affect the performance and small intestine histology parameters in Japanese quail chicks. A total of 160, one-day-old Japanese quail chicks were divided into 4 treatment groups of similar mean weight, comprising 4 subgroups of 10 chicks each. Chicks were fed on their basal diet supplemented by 0, 100, 200 or 400 mg/kg of dietary HPWP for each kg of starter (0 to 10 days), grower (11 to 24 days) and finisher (25 to 42 days) diets for 7 weeks. At the end of 42 days of age, 10 birds per subgroup were slaughtered and intestinal samples were taken to evaluate histomorphological analyses. The results showed that dietary HPWP supplementation did not affect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hot pepper waste powder; Quails; Growth performance; Small intestine; Gut health.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400304
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Effects of glutamine on performance and intestinal mucosa morphometry of broiler chickens vaccinated against coccidiosis Scientia Agricola
Luquetti,Brenda Carla; Alarcon,Miguel Frederico Fernandez; Lunedo,Raquel; Campos,Daniel Mendes Borges; Furlan,Renato Luís; Macari,Marcos.
ABSTRACT This study aimed to assess the effects of glutamine as feed additive on performance and intestinal mucosa morphometry of broiler chickens vaccinated against coccidiosis. A total of 400 day-old male chicks were randomly assigned to four treatments (NVNG – no vaccination, no glutamine supplementation; NVG – no vaccination, glutamine supplementation (10 g kg−1); VNG – vaccination, no glutamine supplementation; VG – vaccination, glutamine supplementation) replicated four times with 25 birds per replicate. A commercial sprayed-on vaccine against coccidiosis containing Eimeria acervulina, E. maxima, E. mivati, and E. tenella was administered at the hatchery. Broiler performance was evaluated from 1-28 days, and morphometric parameters were analyzed at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticoccidial vaccines; Intestinal ephitelium; Gut morphometry; Glutamine supplementation; Small intestine.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400322
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Evaluation of Comparative Effect of Feed Additive of Allium Sativum and Zingeber Officinale on Bird Growth and Histomorphometric Characteristics of Small Intestine in Broilers Rev. Bras. Ciênc. Avic.
Javid,MA; Abbas,G; Waqas,MY; Basit,MA; Asif,M; Akhtar,MS; Masood,S; Saleem,MU; Qamar,SH; Kiani,FA.
ABSTRACT The present study was conducted to evaluate the effects of supplementation of garlic, ginger in the diets of broiler chickens and assessment in terms of feed intake, growth performance and economics of feeding. The results showed that groups supplemented with 0.5% garlic powder and 0.5% ginger powder has shown significant effects on body weight as compared to the control group at day 28. Groups supplemented with 0.5% garlic powder and 0.5% ginger powder show significant increase in body weight than the groups supplemented with 0.25% garlic powder and 0.25% ginger powder. Between different supplemented groups, villus length and width of duodenum and jejunum of birds served with 0.5% garlic powder and 0.5% ginger powder is significantly higher than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ginger; Garlic; Bird performance; Histomorphometry; Small intestine.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200347
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Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats BJMBR
Palanch,A.C.; Alvares,E.P..
Food deprivation has been found to stimulate cell proliferation in the gastric mucosa of suckling rats, whereas the weanling period has been reported to be unresponsive in terms of proliferative activity. In the present study we analyze regional differences in the effect of milk or food deprivation on cell proliferation of the epithelia of the esophagus and of five segments of small intestine in suckling, weanling and newly weaned Wistar rats of both sexes. DNA synthesis was determined using tritiated thymidine to obtain labeling indices (LI); crypt depth and villus height were also determined. Milk deprivation decreased LI by 50% in the esophagus (from 15 to 8.35%) and small intestine (from 40 to 20%) of 14-day-old rats. In 18-day-old rats, milk and food...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Developing rats; Milk deprivation; Cell proliferation; Crypt and villus size; Esophagus; Small intestine.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400015
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Goblet cell number in the ileum of rats denervated during suckling and weaning Biocell
Hernandes,Luzmarina; Marques da Silva Pereira,Lucieni Cristina; Parisi Alvares,Eliana.
The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied at 21 days and rats were killed 15 days after treatment (21/36-day-old) . The number of goblet cells in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benzalkonium chloride; Development; Goblet cells; Myenteric plexus; Small intestine.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300005
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Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition International Journal of Morphology
Franco,Cássia Luiza de Marins; Sant'Ana,Débora de Mello Gonçales; Araújo,Eduardo José de Almeida.
The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4µm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the...
Tipo: Journal article Palavras-chave: Protein-energetic malnutrition; Small intestine; Morphometry; Mucins.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026
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Ischemic post-conditioning attenuates the intestinal injury induced by limb ischemia/reperfusion in rats BJMBR
Leng,Y.F.; Zhang,Y.; Zhang,Y.; Xue,X.; Wang,T.; Kang,Y.Q..
The purpose of this study was to investigate the protective effects of ischemic post-conditioning on damage to the barrier function of the small intestine caused by limb ischemia-reperfusion injury. Male Wistar rats were randomly divided into 3 groups (N = 36 each): sham operated (group S), lower limb ischemia-reperfusion (group LIR), and post-conditioning (group PC). Each group was divided into subgroups (N = 6) according to reperfusion time: immediate (0 h; T1), 1 h (T2), 3 h (T3), 6 h (T4), 12 h (T5), and 24 h (T6). In the PC group, 3 cycles of reperfusion followed by ischemia (each lasting 30 s) were applied immediately. At all reperfusion times (T1-T6), diamine oxidase (DAO), superoxide dismutase (SOD), and myeloperoxidase (MPO) activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Post-conditioning; Limb ischemia-reperfusion injury; Small intestine.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500005
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Modulation of Physiologic Parameters in Molted Isa Brown Laying-Hens by Glutamine + Glutamic Acid Supplementation Rev. Bras. Ciênc. Avic.
Morales,W; Rodríguez,V; Garcia,NV.
ABSTRACT Feed costs are the main limiting factors in poultry industry and alternative sources of food and/or feed supplements to optimize the bird´s life cycle and to extend their production period need to be explored. This study evaluated morphometric parameters of the small intestine and gonadotropin transcript levels in Isa Brown laying hens supplemented with glutamine + glutamic acid (Aminogut®) during a second production cycle. Molting was induced and groups of 100 hens each, were supplemented with 0, 0.8, 1.6 or 2.4% Aminogut® in their diet. At the end of the experimental period, tissue sections from duodenum, jejunum and ileum were processed by the Hematoxylin-Eosin technique and samples from hypothalamus and hypophysis were collected for RT-PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Gonadotropin; Morphometry; RT-PCR; Small intestine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200255
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Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets International Journal of Morphology
Renfeng,Li; Xiangqin,Tian; Songlin,Qiao; Yanyan,Yang; Enmin,Zhou; Gaiping,Zhang.
Microfold (M) cells act as antigen-sampling sites for initiating antigen specific mucosal immune responses, but they may also provide a gateway for enteropathogen entry. In this study we demonstrated villous M cells by morphological and immunohistochemical methods to be present in the three regions of the small intestine from newborn piglets. Immunohistochemical analysis, using anti- cytokeratin 18 (CK18) primary antibodies, showed a gradually decreased density of M cells from the lower crypt epithelium to the upper villous epithelium. The proportion of villous M cells was greater in the ileum than in the duodenum or the mid-jejunum. Ultrastructural observation revealed that villous M cells were mainly columnar in shape in the duodenum and the mid-jejunum,...
Tipo: Journal article Palavras-chave: M Cells; Villous epithelium; Small intestine; Newborn piglets.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400011
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Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration Biocell
Marçal Natali,María Raquel; Molinari,Sonia Lucy; Valentini,Luiz Cristiano; Hubner de Miranda Neto,Marcílio.
The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2) and of the size of the neuronal cell bodies did not reveal statistically significant differences between the groups, but there was a greater incidence of large neurons in the protein restriction group (RP). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myenteric neurons; Small intestine; Morphometric; Hypoproteic ration.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100006
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Probiotics protect the intestinal wall of morphological changes caused by malnutrition Anais da ABC (AABC)
AZEVEDO,JORGE F. DE; HERMES-ULIANA,CATCHIA; LIMA,DIRLENE P.; SANT'ANA,DÉBORA M.G.; ALVES,GILBERTO; ARAÚJO,EDUARDO J.A..
This study sought to morphometrically analyze the jejunal wall of protein-malnourished rats administered a probiotic supplement. The sample consisted of recently weaned Wistar rats (Rattus norvegicus) distributed among four groups: animals given a commercial diet (G1, n = 4); animals given the same ration as G1 plus a probiotic supplement (G2, n = 4); animals given a 4% protein diet (G3, n = 4); and animals given the same ration as G3 plus a probiotic supplement (G4, n = 4). After 12 weeks, part of the jejunum was harvested and subjected to routine histological processing. Transverse sections with a thickness of 3 µm were stained with HE, and histochemical techniques were used to assay for glycoconjugates, including staining with periodic acid-Schiff (PAS)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bowel; Digestive system; Histology; Morphometry; Protein-energy malnutrition; Small intestine.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301303
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The Effect of Early Post Hatch Feeding Times on the Growth and Development of the Gastrointestinal Tract of Mule Ducklings to Five Days of Age Rev. Bras. Ciênc. Avic.
Williams,MA; Lallo,CHO; Sundaram,V.
ABSTRACT The present study evaluated the effects of early post-hatch feeding on the growth and development of the gastrointestinal tract (GIT) of mule ducks reared in the humid tropics to five days of age. A total of 120 newly hatched mule ducklings were selected and randomly assigned to four treatment groups. Each with 30 birds, (T1, T2, T3, and T4) is based on four feeding regimes (feed accessed at 3, 24, 36, and 48 hours post-hatch) for five days. The ducklings were euthanized each day and the parameters: small intestinal samples were processed for histological analysis and histometrical parameters including villi length and width, crypt width and depth, and the number of goblet cells per villus was measured, statistically analyzed using ANOVA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Post hatch feed times; Growth and development; Small intestine; Gastrointestinal tract; Mule ducklings.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100311
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The effects of surgery on gastrointestinal motor activity BJMBR
Quigley,.M.M.; Thompson,J.S..
Gastrointestinal surgical procedures have the potential to disrupt motor activity in various organs of the gastrointestinal tract or, indeed, throughout the entire alimentary canal. Several of these motor effects have important clinical consequences and have also served to advance our understanding of the regulation of gastrointestinal motor activity. This review will focus, in particular, on the effects of surgery on the small intestine, and will attempt to emphasize the implications of these studies for our understanding of small intestinal motility, in general.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Motility; Motor activity; Small intestine; Surgery; Intestinal resection; Intestinal transplantation.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700003
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Upregulation of TLR4 mRNA Expression Levels in Broiler Chickens Under Acute Heat Stress Rev. Bras. Ciênc. Avic.
Huang,S.
ABSTRACT Toll-like receptors (TLRs) are well-characterized in mice and rats, but little is known on their role in broiler chickens during acute heat stress (AHS). The aim of the present study was to estimate the change in TLR4 mRNA expression in the liver, kidney's pleen, heart, and small intestine of broiler chickens under AHS. A total of 240 healthy Arbor Acres (AA) broiler chickens were randomly divided into four groups: control group (22±1°C; 0h), HS2, HS5 and HS10 (38±1°C; 2h, 5h and 10h of heat stress, respectively). As AHS duration increased, TLR4 mRNA expression slightly decreased at HS2 and HS5, but was dramatically elevated in HS10 (p<0.001) compared with the control group in the small intestine, as well as in the spleen at HS2 (p=0.001) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toll-like receptors 4; Liver; Small intestine; Acute heat stress; Broiler chickens.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100087
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